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The Symposium on Prospects in the Physics of Discrete Symmetries is organized by the Physics Department of "Sapienza" University of Rome, and by INFN (Istituto Nazionale di Fisica Nucleare). This second edition of the Symposium, which was successfully held for the first time in Valencia in 2008, covers all theoretical and experimental progress in the field, and aims at a thorough discussion on the latest developments. The Symposium is organized in plenary sessions with invited talks, and parallel sessions with selected contributions from the submitted abstracts. The topics covered include T, C, P, CP symmetries; CPT symmetry, decoherence, Lorentz symmetry breaking; accidental symmetries (B, L conservation); neutrino mass and mixing; cosmology and astroparticles, dark matter searches; experimental prospects at LHC, Super flavor factories, and new facilities.